DNA

Part:BBa_K4765116

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)


Twister P1 + T7_RBS + XRCC1 + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of XRCC1 and ribozyme-assisted polycistronic co-expression system：pRAP. XRCC1 is a vital protein in DNA repair, particularly for single-strand breaks caused by radiation and alkylating agents.

Usage and Biology

We constructed XRCC1 into pRAP to endow E. coli with anti-UV capability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 746
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1796


[edit]
Categories
//collections
Parameters
protein